Preparing Tris Buffer | NCBioNetwork.org

How to Prepare a Tris Buffer Solution Tris-hydroxymethyl aminomethane (TRIS) is widely used for the preparation of buffers in biochemical research. Shown here is the structure of TRIS in its protonated form: ^ NH3 HOCH2- C-CH2OH | CH2OH Its acid dissociation constant, Kα, is 8.32 ×10^-9 M. You have available at your lab bench a 0.1 M solution of TRIS in its protonated form, 0.1 M solutions of HCl and NaOH, and ample distilled water. Describe the preparation of a 1-L soluti

Tris (hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and Kb is 1.19 ×10^-6 for the aqueous reaction (HOCH2)3 CNH2 H2 O ⇌(HOCH2)3 CNH3^ OH^- a. What is the optimal pH for TRIS buffers? b. Calculate the ratio [T R I S] /[T R I S H^ ] at p H=7.00 and at p H=9.00 c. A buffer is prepared by diluting 50.0 g TRIS base and 65.0 g TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L What is the