In this study we explored if LM-031 analogs LMDS-1 to -4 may act as TRKB agonists to protect SH-SY5Y cells against Aβ toxicity. By docking computation for binding with TRKB using 7,8-DHF as a control, all four LMDS compounds displayed potential of binding to domain d5 of TRKB.

Sep 20, 1993 · We demonstrate that SH-SY5Y cells express transcripts encoding the low-affinity NGF receptor (LNGFR) and trkB, the signal transducing receptor unit for BDNF. Interaction of BDNF with SH-SY5Y cells increased the transcription of the c-fos gene, showing that these molecules encode functional BDNF receptors.

Apr 8, 2021 · Based on the observation that SH-SY5Y cells express TrkB and p75 NTR receptors, we investigated whether BDNF and its low molecular weight mimetic protect SH-SY5Y cells from serum withdrawal.

Different pathways are involved in 5-HT7 receptor-induced increases in TrkB expression in SH-SY5Y cells and RGC-5 cells. SH-SY5Y cells were pre-treated with 10 μM H89 (A) or 30 μM rhosin (B) for 30 min prior to the addition of 300 nM LP 12 for 2 additional h.

Jun 1, 2003 · The RA-treated SH-SY5Y cells expressing trkB are highly proliferating in the presence of BDNF and serum, and the BDNF stimulation also enhances a migratory response (Matsumoto et al, 1995).

May 1, 2019 · The molecular mechanism on Trx-1 expression induced by BDNF through TrkB/Akt/CREB pathway in SH-SY5Y cells. BDNF binds and activates its receptor TrkB, then triggers PI3-K/Akt/CREB signal pathway. Finally, BDNF induces Trx-1 expression.

Sep 15, 2003 · RA induces TrkB expression and confers APP promoter responsiveness to BDNF in SH-SY5Y cells. TrkB levels (messenger and cellular protein) were analyzed after 4 days of incubation in the presence or the absence of 1 μM RA.

Sep 1, 2020 · We applied two-step retinoic acid (RA) and brain-derived neurotrophic factor (BDNF) protocol to monitor the conversion of undifferentiated SH-SY5Y into neuron-like cells with distinctly polarized axon-dendritic morphology and formation of bona fide synaptic connections.

Oct 1, 2008 · Expression of XRCC4, a central component of NHEJ, was significantly upregulated in SY5Y-TrkA compared to SY5Y-TrkB cells. Expression data were confirmed using real-time PCR and western blotting. Additionally, XRCC4 expression was enhanced in most primary neuroblastomas with high TrkA expression.

SY5Y-TrkB (TrkB) and control wild-type SY5Y (wt) cells were studied. A heterogeneous protein containing phosphotyrosine, consistent with the relative gel mobility (M r ) of TrkB, is recognized only for SY5Y-TrkB cells activated by BDNF or mAb 1D7.