Compared to their predecessors (Tet-On and Tet-On Advanced systems), Tet-On 3G systems demonstrate lower basal expression (by 5- to 20-fold) and higher sensitivity to doxycycline (Dox) induction, which is particularly advantageous for in vivo studies in tissues where high Dox concentrations are difficult to attain (e.g., brain). We offer ...

The Tet-On 3G Systems are inducible gene expression systems for mammalian cells. Target cells that Target cells that express the Tet-On 3G transactivator protein and contain a gene of interest (GOI) under the control of a

Tet-On 3G is the gold standard tetracycline-inducible expression system for mammalian cells, generating the lowest basal expression and highest fold-induction.

Lenti-X™ Tet-On® 3G Inducible Expression System User Manual (030420) takarabio.com Takara Bio USA, Inc. Page 3 of 24 I. Introduction A. Summary The Tet-On 3G Systems are inducible gene expression systems for mammalian cells. Target cells that express the Tet-On 3G transactivator protein and contain a gene of interest (GOI) under the

Based on the transcriptional regulators described by Gossen and Bujard, 1992; Gossen et al. 1995; and Urlinger et al. 2000; Tet-On 3G is a modified form of the Tet-Advanced transactivator protein which has been altered to display far higher sensitivity to doxycycline (Dox) (Zhou et …

CHO Tet-On 3G is a an epithelial-like cell line derived from Chinese hamster ovary (CHO-K1 clone) that expresses the tetracycline (Tet)-regulated transactivator Tet-On 3G. Inducible expression of any gene can be achieved by transfecting or transducing this cell line with a vector containing your gene of interest under the control of a ...

Tet-On 3G is a modified, improved Tet-On Advanced transcriptional activator protein (transactivator) which has been evolved to display far higher sensitivity to doxycycline. Tet-On 3G generates very high maximal expression and responds to lower Dox …

The Lenti-X Tet-On 3G Inducible Expression System (EF1alpha, mCherry) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system in both dividing and non-dividing cell types. pLVX-TRE3G ...

All plasmid-based versions of the Tet-On 3G System contain the following seven components: 10 μg regulator plasmid ( see tables below ) 10 μg response plasmid ( see tables below )

Third generation: Tet-On 3G systems. The Tet-On 3G system is the most advanced inducible expression system for mammalian cells. The transactivator is similar to that of the Tet-On Advanced system but additional mutations lead to further sensitivity to doxycycline.