Sortase A (EC 3.4.22.70, SrtA, SrtA protein, SrtA sortase) is an enzyme. [ 1 ] [ 2 ] [ 3 ] This enzyme catalyses a cell wall sorting reaction, in which a surface protein with a sorting signal containing a LPXTG motif, is cleaved between the Thr and Gly residue.

Sortase refers to a group of prokaryotic enzymes that modify surface proteins by recognizing and cleaving a carboxyl-terminal sorting signal.

Jul 1, 2024 · Sortases are a widespread family of bacterial transpeptidases that covalently anchor proteins to the cell surface and assemble bacterial pili.

Sortase A, a transpeptidase enzyme is present in many Gram-positive bacteria and helps in the recruitment of the cell surface proteins. Over the last two decades, Sortase A has become an attractive tool for performing in vivo and in vitro ligations.

We focus primarily on the transpeptidase sortase A, which has become popular over the past few years for its ability to perform a remarkably wide variety of protein modifications, both in vitro and in living cells.

Sortase A, a housekeeping transpeptidase from Staphylococcus aureus, catalyzes the covalent anchoring of surface proteins to the cell wall. It links the threonyl carboxylate of an LPXTG recognition motif to the amino group of the pentaglycine peptide attached to a peptidoglycan.

Jan 11, 2021 · Here, we describe the laboratory evolution of the bacterial transpeptidase sortase A to recognize the LMVGG sequence in endogenous amyloid-β (Aβ) protein.

Dec 4, 2020 · As a well-studied member of the sortase subfamily catalysing the cell wall anchoring of important virulence factors to the surface of staphylococci, enterococci and streptococci, sortase A plays a critical role in Gram-positive bacterial pathogenesis.

Oct 6, 2018 · We focus primarily on the transpeptidase sortase A, which has become popular over the past few years for its ability to perform a remarkably wide variety of protein modifications, both in vitro and in living cells.

Sortase A (SrtA, EC number: 3.4.22.70) is a membrane-anchored transpeptidase first found in Staphylococcus aureus, which anchors surface proteins to cell wall by a cell-wall sorting reaction (Mazmanian et al. 1999).