The IFN-induced double-stranded RNA-dependent protein kinase (PKR) is one of the four mammalian serine-threonine kinases (the three others being HRI, GCN2 and PERK) that phosphorylate the eIF2 alpha translation initiation factor, in response to stress signals, mainly as a result of viral infections. eIF2 alpha phosphorylation results in arrest o...

Aug 29, 2008 · Upon binding double-stranded RNA (dsRNA), PKR undergoes autophosphorylation reactions that activate the kinase. PKR then phosphorylates eukaryotic initiation factor 2alpha, thus inhibiting protein synthesis in virally infected cells.

PKR (protein kinase R) is a central component of the interferon antiviral defense pathway. Upon binding dsRNA, PKR undergoes autophosphorylation reactions that activate the kinase. PKR then phosphorylates eIF2α, thus inhibiting protein synthesis in virally-infected cells.

PKR binds the HCV IRES RNA, cooperates with some functions of the HCV core protein and may represent a target for NS5A or E2. Novel data points out for a role of PKR as a pro-HCV agent, both as an adapter protein and as an eIF2α-kinase, and in …

Jun 1, 2007 · PKR is activated in response to dsRNA of cellular, viral, or synthetic (such as polyrI:polyrC, pIC) origin, with a size greater than 30 nucleotides. PKR mediates a critical role in response to dsRNA, acting as a sensor of viral infections.

Here, we show that dsRNA induces the formation of a novel condensate by PKR that is distinct from other known ribonucleoprotein assemblies. These results challenge prior observations that PKR is recruited to stress granules and suggest that the condensation of PKR may be a mechanism that cells use to modulate PKR activation.

dsRNA-dependent protein kinase R (PKR) is a ubiquitously expressed enzyme well known for its roles in immune response. Upon binding to viral dsRNA, PKR undergoes autophosphorylation, and the phosphorylated PKR (pPKR) regulates translation and multiple signaling pathways in …

Apr 22, 2005 · A key player in these dsRNA-mediated events is PKR (protein kinase RNA-dependent), an IFN-inducible serine/threonine protein kinase that autophosphorylates following association with dsRNA...

Jun 13, 2017 · Here, we demonstrate that single-stranded RNAs bind to PKR with micromolar dissociation constants and can induce activation. Addition of a 5′-triphosphate slightly enhances binding affinity.

Aug 15, 2022 · However, intracellular long dsRNA was sensed by PKR and TLR3, which activate distinct signals, and synergistically induced rapid apoptosis.