Guide RNA (gRNA) or single guide RNA (sgRNA) is a short sequence of RNA that functions as a guide for the Cas9-endonuclease or other Cas-proteins [1] that cut the double-stranded DNA and thereby can be used for gene editing. [2]

This year’s Nobel prize for the CRISPR/Cas system is an illustrative example of how scientific breakthroughs rests on preceding work: the discovery of guide RNAs in the 1990s. Subject Categories: S&S: History & Philosophy of Science, RNA Biology.

Nov 5, 2020 · The team teased out some guidelines for guide RNA design, which led to their Rule Set 1 and Rule Set 2 — quantitative models to assess sgRNA on-target specificity.

Guide RNA refers to a RNA molecule that contains an anchor sequence and is used to direct the editing of preedited RNA by hybridizing with it and guiding the addition or deletion of nucleotides as directed by the template.

Feb 27, 2025 · Zhang’s team discovered more than 20,000 different Tas proteins, mostly occurring in bacteria-infecting viruses. Sequences within each gene’s repetitive region — its TIGR arrays — encode an RNA guide that interacts with the RNA-binding part of the protein. In some, the RNA-binding region is adjacent to a DNA-cutting part of the protein.

Feb 26, 2025 · We present GuideScan2 for memory-efficient, parallelizable construction of high-specificity CRISPR guide RNA (gRNA) databases and user-friendly design and analysis of individual gRNAs and gRNA libraries for targeting coding and non-coding regions in custom genomes. GuideScan2 analysis identifies widespread confounding effects of low-specificity gRNAs in published CRISPR screens and enables ...

Dec 1, 2020 · The Cas9 cleavage site is determined by a small RNA molecule, termed guide RNA (gRNA), that base pairs with the target DNA and at the same time binds to Cas9 to initiate the site‐specific double‐strand break (Fig. 1).

2.1 Guide RNA design & delivery. The guide RNA consists of two components: a crispr RNA (crRNA) and tracrRNA. The crRNA is a unique sequence that confers genomic targeting, and the tracrRNA is a scaffold RNA common to all guides.

Cas13 proteins are guided to their target RNAs by a single CRISPR RNA (crRNA) composed of a direct repeat (DR) stem loop and a spacer sequence (guide RNA or gRNA) that mediates target recognition by RNA-RNA hybridization.

Oct 13, 2020 · Here, we present dbGuide (https://sgrnascorer.cancer.gov/dbguide), a database of functionally validated guide RNA sequences for CRISPR/Cas9-based knockout in human and mouse.