Mature form of CD30 protein is processed from a precursor weighing 84 kD and during transit in the Golgi complex gains weight through glycosylation 3 domains: Extracellular (361 amino acids)

In IHC, tumor cells strongly express CD30 (membranous and Golgi patterns). Further, CD20 is negative, and PAX5 is weakly positive for these cells ( Fig. 5 ). CD30 staining has an extremely high sensitivity, so it could be said that CD30 is positive in almost all cHL tumor cells.

Jan 26, 2024 · Fix tissue blocks (less than 1 cm thick) in 10% formalin for 24 hours or more. The alternatively you can also use the above fixative solution: 2. Place tissue blocks in 3% potassium bichromate for 3-7 days. Avoid light. Change fresh solution each day. 3. Transfer tissue blocks into 2% silver nitrate solution for 1-3 day at room temperature.

Mar 5, 2024 · The Golgi apparatus (also called the Golgi body, Golgi complex, or dictyosome) is an organelle found in typical eukaryotic cells. It was identified in 1898 by the Italian physician Camillo Golgi and was named after him.

The Golgi apparatus plays a central role in normal cell physiology by promoting cell survival, facilitating proliferation, and enabling cell-cell communication and migration. These roles are partially mediated by well-known Golgi functions, ...

Oct 19, 2021 · Immunohistochemical stains show the atypical cells are positive for CD30 and CD15. Both show prominent Golgi area staining. EBER staining by in-situ hybridization was positive and restricted to the atypical cells. Pax5 was also positive in the large cells, but shows weak expression.

Sep 1, 2015 · Golgi phosphoprotein3 (GOLPH3) is known as an oncoprotein and may be a prognostic biomarker in various tumors. Here we performed a meta-analysis on the association of GOLPH3 expression and survival in solid tumors.

The Golgi-Cox Kit is designed for the staining of neuronal dendrites and dendritic spines on fresh or paraformaldehyde fixed brain tissues. The Kit has been extensively tested on both fresh and paraformaldehyde fixed brain tissues in rats and mice and should work on other species as well.

Nov 19, 2021 · Golgi membrane protein 1 (GOLM1) is correlated to hepatocellular carcinoma (HCC) progression and metastasis. However, little is known about the role of GOLM1 in regulating the...

Sep 25, 2018 · In this pilot study, we compared two common methods of heat-induced epitope retrieval (HIER) including microwave oven and autoclave, on the quantity and intensity of CD15 and CD30 IHC staining. Study design.