Acidified samples, one from each replicate set, were thawed and vortexed prior to analysis for aqueous Mn, Ni, and Na concentrations by inductively coupled plasma-optical emission spectroscopy ...

The frozen sample stored at -80°C was thawed on ice. A 400 μL solution (Methanol: Water = 7:3, v/v) containing an internal standard was added to a 20 mg sample and vortexed for 3 min. Subsequently, ...

For DNA complex formation, 25 kDa branched polyethylenimine (bPEI; Sigma-Aldrich) was dissolved in reduced serum medium OptiMEM (Fisher Scientific) and then added dropwise to DNA in OptiMEM, vortexed ...

The solution was gently heated in a 40°C water bath and vortexed every 30 s until the solution was clear. ISRIB was injected intraperitoneally after tibial fracture surgery in mice, the dosage we gave ...

Total faecal DNA was extracted from the supernatant using QIAamp DNA Stool Mini Kit (Qiagen). Two vaginal swabs were vortexed vigorously in 0.5 mL PBS and 0.5 mL Qiagen’s AL buffer for 2 min to ...

A sample was suspended in Phosphate Buffered Saline (PBS) 0.1M, pH 7.4, in a 1:1 proportion. The suspensions were vortexed and subjected to three freeze and thaw cycles to release the genetic material ...

The mixture was vortexed for 10 min, followed by centrifugation at 2,500 g for 10 min. An aliquot of 100 μL supernatant was transferred to another clean 96-well plate containing 100 μL ...

Abstract Rare and common genetic variants contribute to the risk of atrial fibrillation (AF). Although ion channels were among the first AF candidate genes identified, rare loss-of-function variants ...

Structural Genomics Consortium, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States Department of Pharmacology, University of ...