Mouse antihuman CD144 (1:100, 555661; BD Pharmingen) was used as the primary antibody, followed by Hoechst (1:2000, H3569; Invitrogen), Phalloidin–Tetramethylrhodamine B isothiocyanate (1:200, P1951; ...
Nuclei were stained with DAPI (blue). CAMSAP2 was stained with rabbit anti-CAMSAP2 antibody (green). F-actin was stained with phalloidin (white). We fixed the cells after 2 hours of migration with ...
The flavonoid silymarin and one of its structural components, silibinin, are substances with documented hepatoprotective properties. Their mechanisms of action are still poorly understood. However ...