For optimal sensitivity and specificity, design of a nested PCR and a magnetic capture step of the first PCR product (LTR primer is biotinylated) is recommended.

PCR-Machines are classified depending on the capacity of PCR used such as Nested PCR or Reverse Transcriptase PCR (RT-PCR ), or Multiplex RT PCR.

One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the most widely applied method for COVID-19 diagnostics. Notwithstanding the facts that one-step qRT-PCR is well ...

Second, more practical details about the tool itself would be very helpful. Simply stating that it is available on GitHub may not be enough. Readers will want to know what programming language it uses ...