By contrast, in the present case, there is good agreement between ΔΔG exp ⧧ and ΔΔG calc ⧧ for only the Q295A variant of hlGPDH, and large differences (ΔG calc ⧧ – ΔG exp ⧧) = ΔΔG calc→exp ⧧ of −3.8 ...