Frontiers11d
Study on the efficacy of IFN-γ- and sPD-1-overexpressing BMSCs in enhancing immune effects for the treatment of lung adenocarcinoma
The mRNA levels of IFN-γ and sPD-1 in BMSCs were verified by qPCR, telomerase activity was measured following ... Peripheral blood (100 μL per tube) was stained with CD4 and CD25 for surface marker ...
technologynetworks6d
Streamlining Single-Cell Analysis With Automated Tissue Dissociation
Advancing single-cell research requires precise and reproducible tissue dissociation techniques. Manual methods can introduce variability, compromise cell viability and hinder downstream applications.
Frontiers27d
Cannabinoid receptor 2 selective agonist ameliorates adjuvant-induced arthritis by modulating the balance between Treg and Th17 cells
Quantitative PCR (qPCR) was performed using SYBR Green dye (Vazyme, Q711-02) on a ViiA7 PCR system (Applied Biosystems) in 384-well plates, with each reaction run in triplicate to ensure accuracy. The ...
eLife12d
CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cells
This study presents important findings on the role of CXXC-finger protein 1 in regulatory T cell gene regulation and function. The evidence supporting the authors' claims is convincing, with mostly ...
gut.bmj25d
Integrated multiomic analysis reveals comprehensive tumour heterogeneity and novel immunophenotypic classification in hepatocellular carcinomas
7 Center for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, China Objective Hepatocellular carcinoma (HCC) is heterogeneous, especially in multifocal tumours, ...
gut.bmj14d
NAD metabolism fuels human and mouse intestinal inflammation
4 Institute of Legal Medicine and Core Facility Metabolomics, Medical University Innsbruck, Innsbruck, Austria Objective Nicotinamide phosphoribosyltransferase (NAMPT, also referred to as pre-B cell ...