Stimulated Emission Depletion (STED) microscopy is a super-resolution imaging technique that overcomes the diffraction limit of conventional optical microscopy. It enables the visualization of ...

STED microscopy overcomes this barrier, achieving resolution up to 10 times better than conventional methods. STED microscopy ...

Leica Microsystems now sells an STED microscope with a resolution of 80 nm. At present, the far-field methods are those chosen for imaging structures below a surface, such as inside a cell.

Nanoscopy techniques aim to break this diffraction barrier and achieve super-resolution imaging. STED microscopy uses a doughnut-shaped depletion laser to selectively suppress the fluorescence ...

Our gated 3D STED system enables super-resolution imaging of some green and red fluorophores ... conventional TIRF imaging by our Abbelight SAFe360 system on Olympus IX83 microscope.

and retains its fluoresescence under the STED microscope for a very long time. This video was shot at 1.5 fps and a resolution of 90nm. Still images were captured at 60nm resolution. In response ...

Inspired by a super-resolution microscopy technique known as STED, we are showing that it is indeed possible to write features whose dimensions are substantially smaller than the diffraction limit.

super-resolution imaging approaches: instant structured illumination microscopy, or iSIM, and stimulated emission depletion, or STED. Because each sample and each research project is unique, it would ...