The clever use of a specially modified primer during PCR allows the rapid and inexpensive gel electrophoresis-based purification of single-stranded DNAs. Single-stranded DNA (ssDNA) molecules, which ...
(A) RFs slow and then stall during HU treatment, generating ssDNA. (B) This ssDNA is a signal that resembles DNA damage from resected DNA DSBs or single-strand repair patches. (C) ssDNA is also ...
Detection of ssDNA quantities as low as 60 femtomoles is possible by eye alone. For sequence-specific detection of DNA in a sample such as that from a PCR reaction, short ssDNA probes are added to ...
At a GMP-compatible scale, the KI efficiency of GenExactâ„¢ ssDNA through electroporation ... efficiency Comparing GenWandTM dsDNA to internal PCR dsDNA (38% vs. 21%), the KI efficiency was ...