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In fact, microarray analysis enables researchers ... first isolate mRNA from a target sample, convert it into complementary DNA (cDNA), and label the cDNA with a fluorescent dye.
The DNA copy that is made, called complementary DNA (cDNA), is then applied to the microarray. The cDNA binds to complementary base pairs in each of the spots on the array, a process known as ...
The basic principle behind the DNA microarray is “nucleic acid hybridization”. In this process, two complementary strands ... excites fluorescence of the cDNA, generating signals.
The 'sequence' approach has led to the discovery of a wide variety of sequence motifs encoding structural domains, such as DNA–binding ... technical aspects of cDNA microarrays, although some ...
Serial analysis of gene expression (SAGE) uses mRNA from a sample to create complementary DNA (cDNA ... to a solid support. The microarray is assembled by spotting cDNA samples onto a solid ...
4 They used “Lymphochip” complementary DNA (cDNA) microarrays, covering a broad range of genes expressed by lymphoid cells and others suspected of being involved in cancer and immunology, to compare ...
For gene-expression profiling, each element usually represents one gene and is created with the use of a complementary DNA (cDNA ... The use of DNA microarrays has been suggested for other ...
The development of genome analysis technologies such as DNA microarrays and next generation sequencing (NGS) has provided the researcher with the unique ability to screen for sequence variants of ...
The new DNA that's created from the mRNA is called cDNA (see DNA to cDNA schematic). Just like pre-RNA is a complementary copy of the DNA template, the cDNA is a complementary copy of the mRNA ...
However, the court found cDNA (complementary DNA) to be patentable. The meaning of this ruling is complicated significantly by the court’s sketchy understanding of molecular biology. Justice ...