One of the most surprising revelations from genomics, and since validated by the Human Genome Project, was that only 4% of the human genome codes for protein. At one time molecular biologists ...

Prior to the invention of RNA-seq, gene expression microarrays were used to analyze the transcriptome. Now, with the invention of NGS, RNA-seq has many benefits over gene expression arrays, including: ...

While DNA microarrays are already widely used, adapting the technology to RNA microarrays has proved difficult due to the lower stability of RNA. In 2018, the University of Vienna demonstrated how ...

For 20 years, DNA microarrays have been the go-to method for revealing patterns of gene expression. But as the price of next-generation sequencing continues to fall, RNA sequencing (RNA-seq) has ...

8 RNA-seq is widely regarded as superior to other technologies, such as microarray hybridization. There are several reasons for RNA-seq’s well-regarded status: Not limited to genomic sequences – ...

Microarrays are built upon the concept of hybridization, the binding of cDNA, complementary DNA strands derived from sample RNA, to strands of synthetic DNA. In the prototypic microarray ...

One way they do this is to use a DNA microarray to determine the expression levels of genes. When a gene is expressed in a cell, it generates messenger RNA (mRNA). Overexpressed genes generate ...

The concordance of RNA-sequencing (RNA-seq) with microarrays for genome-wide analysis of differential gene expression has not been rigorously assessed using a range of chemical treatment conditions.

Central to microarray analysis for determining gene expression is the hybridization of fluorescently labeled RNA with a short strand of complementary DNA that is tethered to a solid surface ...

We sought to develop and apply a variation of traditional RNA-SIP, one that would combine ... With Chip-SIP, we use a high-density microarray to phylogenetically sort the sample into individual ...