Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

Flow cytometry is the basic component of multicolor flow cytometry. In essence, the procedure has been designed to simultaneously analyze thousands of cells across multiple parameters, doing so in ...

However, with specific needs for each experiment and the addition of sorting, mass spectrometry and imaging, the field of flow cytometry is becoming more and more complex. Download this app note to ...

While flow cytometry can reliably assess surface and intracellular marker expression ... and a CD4 receptor detection antibody were used along with intracellular staining to determine FOXO1 ...

Flow cytometry is a crucial technique for many scientists because it allows them to rapidly detect, sort and analyse cells with particular characteristics. But traditional flow cytometry can be a ...

A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods 139, 271–279 (1991). Article CAS Google Scholar ...

In addition, photostability is an essential dye characteristic for fluorescence microscopy, while choosing brighter dyes is generally beneficial for flow cytometry. Finally, consider the experimental ...

Flow cytometry and cytogenetics are often used in combination for full diagnosis and prognosis of a disorder. ... A number of staining techniques can be used to highlight the regions of interest, ...

Flow cytometry is a high throughput quantitative application that employs fluorescent-labeled molecules for multi-parametric analysis of millions of individual cells or particles, providing ...