Acidification of the endosomal pathway is important for ligand and receptor sorting, toxin activation, and protein degradation by lysosomal acid hydrolases. Fluorescent probes and imaging methods were ...

Improved culture conditions have been developed that will support clonal growth of Swiss mouse embryo 3T3 cells at concentrations of serum protein as low as 125 µ g/ml. Survival of the cells under ...

Since Swiss 3T3 are difficult to transfect, and high expression rates of HA-Rap1 are required for the biochemical pulldown assay, COS-7 cells were used for further characterization.