(Image: Adapted from PicoQuant) In STED microscopy, the sample is labeled with fluorescent dyes or probes that can be excited by a specific wavelength of light. When excited, these fluorophores emit ...

Inspired by a super-resolution microscopy technique known as STED, we are showing that it is indeed possible to write features whose dimensions are substantially smaller than the diffraction limit.

the maximum energy delivered by STED sets the shortest lifetime in the sample while the minimum STED energy (at the center of the donut) marks the longest lifetime, where fluorophores undergo the ...

STED microscopy uses small fluorescent particles—fluorophores—in the sample that glow (fluorescence) with the help of an excitation laser. A second laser beam with a donut-shaped cross-section ...