Simple assembly reactions—as, for example, those used to clone a single PCR fragment into a recipient vector—can be performed in 5 minutes with cloning efficiencies higher than 97% (Figure 2C).

Agilent’s AffinityScript One-Step RT-PCR kit is a high-performance system for high-throughput RT-PCR in a one-step reaction. ... Agilent Launches One-Step Setup RT-PCR Kit for Cloning, Gene Expression ...

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

Heredity - PCR cloning, it is still an issue. Each chapter is written as an independent article, with an Introduction, Materials and methods, and Notes that provide additional technical information.

PCR ‘cloning’ process is not the same as human cloning The video refers to a paper published in the Journal of Biological Engineering in 2015. This paper looked at how the PCR method could be used for ...

Here, we describe a method of systematic PCR screening with multiround sample pooling for the isolation of rare PCR-positive samples. As an example, we have applied this protocol to the recovery ...

My cry of frustration echoed down the hall. My latest attempt to subclone five important PCR products had failed yet again. After two months of fruitless effort, I was at my wit's end until a ...

Additionally, PCR-based strategies have been hampered by laborious cloning steps and low efficiencies (e.g., long range PCR of vector backbones); a contrast to the site-directed method’s >80% ...

Polymerase chain reaction (PCR) test kits for COVID-19 use what’s known as gene cloning – not reproductive cloning – to detect the presence or absence of the SARS-CoV-2 virus, but social ...

Agilent Technologies Inc. has announced availability of the AffinityScript One-Step RT-PCR Kit. The launch of the AffinityScript One-Step RT-PCR Kit represents a re-entry into the one-step RT-PCR ...