Credit: Courtesy of The Smithsonian Institution The "aha" moment and initial experiments in 1983 through which Kary Mullis developed the idea of polymerase chain reaction (PCR) are a well trodden ...

What is PCR? The key element of PCR is heat. Throughout the PCR process, DNA is subjected to repeated heating and cooling cycles during which important chemical reactions occur. During these ...

PCR amplifies DNA in a three-step process ... the single strands starting at the primer to replicate the DNA. The cycle repeats and copies of the original DNA accumulate exponentially.

The mixture is then placed in an RT–PCR machine. The machine cycles through temperatures that heat and cool the mixture to trigger specific chemical reactions that create new, identical copies of the ...

Following synthesis and at the end of the first cycle, each double-stranded DNA molecule consists of one new and one old DNA strand. PCR then continues with additional cycles that repeat the ...

It degraded at 94°C, the temperature required to denature the DNA target; this meant that the scientists had to open the PCR reaction tube at the start of every cycle to add fresh enzyme. Additionally ...

The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features ...