or in the case of multiplexed RT-PCR, lack viral sequence information. Next-generation sequencing (NGS) hybrid capture combines high-throughput capabilities with high sensitivity, helping scientists ...

The Genomics Core offers a wide range of DNA library preps from whole-genome PCR free to amplicon sequencing and CRISPR ... Included in the cost of an NGS DNA library prep: DNA Quality Control - Qubit ...

Advances in next-generation sequencing (NGS) technology have improved ... including required PCR cycles. The Illumina MiSeq system is used to perform sequencing. Figure 2. Graphical representation ...

NGS uses a single tube reaction, taking advantage of the conserved sequences on the 5´ and 3´ ends of each viral RNA. PCR products are rather large, but by employing the Nextera Tagmentation ...

NGS is still predominantly used in a research ... [37] To achieve this, the selected target regions must be PCR amplified, quantified and pooled in equimolar amounts, hereby generating target ...