The enzyme buffer should be prepared in advance. We usually make 250–500 mL of buffer at a time. The buffer will stay good for years if stored in a glass bottle.

After being diluted with media or a buffer, proteolytic enzymes can undergo autolysis. Thus it is recommended that enzymes be dissolved immediately before use and stored on ice (2–8°C).

This study investigated the effects of lanthanides on alkaline phosphatase activity, a model enzyme essential for phosphate metabolism. Reactions were conducted in 96-well microplates using a 0.1 M ...

A study of the conditions favoring growth and toxin production in differently prepared cultures of a toxicogenic strain of the diphtheria bacillus has indicated that toxin is not accumulated unless ...