Blocking G i protein–coupled receptor signaling by pertussis toxin as well as deficiencies in Gα i2, chemokine receptor 2 (CCR2), CCR5, chemokine ligand 2 (CCL2, also known as monocyte ...

To measure CCL2 in highly enriched neuronal culture supernatants, ELISA plates (96-well) were coated with corresponding mouse anti-human antibodies (1–2 μg/ml) overnight at 4°C. The plates ...

In the end, they gathered a cohort of nine children who had similar lung phenotypes and interesting mutations in CCR2. Most of the children’s variants were in the gene’s transmembrane domain.